equal protein loading Search Results


bca protein assay kit  (Thermo Fisher)
99
Thermo Fisher bca protein assay kit
Bca Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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silver staining kit  (FUJIFILM)
90
FUJIFILM silver staining kit
Silver Staining Kit, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nitrocellulose membranes  (Bio-Rad)
99
Bio-Rad nitrocellulose membranes
Nitrocellulose Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sds page gel  (Bio-Rad)
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Bio-Rad sds page gel
Sds Page Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mini protean tgx gels  (Bio-Rad)
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Bio-Rad mini protean tgx gels
Mini Protean Tgx Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal antibody against gapdh 6c5  (Danaher Inc)
99
Danaher Inc mouse monoclonal antibody against gapdh 6c5
Figure 1. Western blots showing the positive and negative controls of NOXs 1-4 antibodies. Autoradiographs showing total NOX1 (A), NOX3 (C) and NOX4 (D) densities in cardiac ventricles from rats and normal hamsters (NH) as well as in A10 cells. In panel B, the autoradiograph shows the total NOX2 density at the cardiac ventricle level in NHs as well as in A10 cells. In panel D, HK cell extracts were used as positive controls for the NOX4 antibody. Negative controls were performed by incubating the membranes in the presence of the respective antibody blocked with the corresponding NOX peptide from the supplier. The band corresponding to the reference protein <t>GAPDH</t> shows the presence of proteins in the negative control wells.
Mouse Monoclonal Antibody Against Gapdh 6c5, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal antibody against gapdh 6c5 - by Bioz Stars, 2026-06
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protein concentration  (Bio-Rad)
99
Bio-Rad protein concentration
Figure 1. Western blots showing the positive and negative controls of NOXs 1-4 antibodies. Autoradiographs showing total NOX1 (A), NOX3 (C) and NOX4 (D) densities in cardiac ventricles from rats and normal hamsters (NH) as well as in A10 cells. In panel B, the autoradiograph shows the total NOX2 density at the cardiac ventricle level in NHs as well as in A10 cells. In panel D, HK cell extracts were used as positive controls for the NOX4 antibody. Negative controls were performed by incubating the membranes in the presence of the respective antibody blocked with the corresponding NOX peptide from the supplier. The band corresponding to the reference protein <t>GAPDH</t> shows the presence of proteins in the negative control wells.
Protein Concentration, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein concentration - by Bioz Stars, 2026-06
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equal protein loading  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc equal protein loading
Figure 1. Western blots showing the positive and negative controls of NOXs 1-4 antibodies. Autoradiographs showing total NOX1 (A), NOX3 (C) and NOX4 (D) densities in cardiac ventricles from rats and normal hamsters (NH) as well as in A10 cells. In panel B, the autoradiograph shows the total NOX2 density at the cardiac ventricle level in NHs as well as in A10 cells. In panel D, HK cell extracts were used as positive controls for the NOX4 antibody. Negative controls were performed by incubating the membranes in the presence of the respective antibody blocked with the corresponding NOX peptide from the supplier. The band corresponding to the reference protein <t>GAPDH</t> shows the presence of proteins in the negative control wells.
Equal Protein Loading, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris glycine sodium dodecyl sulfate polyacrylamide gel electrophoresis  (Bio-Rad)
98
Bio-Rad tris glycine sodium dodecyl sulfate polyacrylamide gel electrophoresis
Figure 1. Western blots showing the positive and negative controls of NOXs 1-4 antibodies. Autoradiographs showing total NOX1 (A), NOX3 (C) and NOX4 (D) densities in cardiac ventricles from rats and normal hamsters (NH) as well as in A10 cells. In panel B, the autoradiograph shows the total NOX2 density at the cardiac ventricle level in NHs as well as in A10 cells. In panel D, HK cell extracts were used as positive controls for the NOX4 antibody. Negative controls were performed by incubating the membranes in the presence of the respective antibody blocked with the corresponding NOX peptide from the supplier. The band corresponding to the reference protein <t>GAPDH</t> shows the presence of proteins in the negative control wells.
Tris Glycine Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein loading buffer p1016  (Beijing Solarbio Science)
90
Beijing Solarbio Science protein loading buffer p1016
Figure 1. Western blots showing the positive and negative controls of NOXs 1-4 antibodies. Autoradiographs showing total NOX1 (A), NOX3 (C) and NOX4 (D) densities in cardiac ventricles from rats and normal hamsters (NH) as well as in A10 cells. In panel B, the autoradiograph shows the total NOX2 density at the cardiac ventricle level in NHs as well as in A10 cells. In panel D, HK cell extracts were used as positive controls for the NOX4 antibody. Negative controls were performed by incubating the membranes in the presence of the respective antibody blocked with the corresponding NOX peptide from the supplier. The band corresponding to the reference protein <t>GAPDH</t> shows the presence of proteins in the negative control wells.
Protein Loading Buffer P1016, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sodium dodecyl sulfate sds  (Bio-Rad)
97
Bio-Rad sodium dodecyl sulfate sds
Figure 1. Western blots showing the positive and negative controls of NOXs 1-4 antibodies. Autoradiographs showing total NOX1 (A), NOX3 (C) and NOX4 (D) densities in cardiac ventricles from rats and normal hamsters (NH) as well as in A10 cells. In panel B, the autoradiograph shows the total NOX2 density at the cardiac ventricle level in NHs as well as in A10 cells. In panel D, HK cell extracts were used as positive controls for the NOX4 antibody. Negative controls were performed by incubating the membranes in the presence of the respective antibody blocked with the corresponding NOX peptide from the supplier. The band corresponding to the reference protein <t>GAPDH</t> shows the presence of proteins in the negative control wells.
Sodium Dodecyl Sulfate Sds, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. Western blots showing the positive and negative controls of NOXs 1-4 antibodies. Autoradiographs showing total NOX1 (A), NOX3 (C) and NOX4 (D) densities in cardiac ventricles from rats and normal hamsters (NH) as well as in A10 cells. In panel B, the autoradiograph shows the total NOX2 density at the cardiac ventricle level in NHs as well as in A10 cells. In panel D, HK cell extracts were used as positive controls for the NOX4 antibody. Negative controls were performed by incubating the membranes in the presence of the respective antibody blocked with the corresponding NOX peptide from the supplier. The band corresponding to the reference protein GAPDH shows the presence of proteins in the negative control wells.

Journal: Canadian journal of physiology and pharmacology

Article Title: Increase of NADPH oxidase 3 in heart failure of hereditary cardiomyopathy 1 .

doi: 10.1139/cjpp-2019-0055

Figure Lengend Snippet: Figure 1. Western blots showing the positive and negative controls of NOXs 1-4 antibodies. Autoradiographs showing total NOX1 (A), NOX3 (C) and NOX4 (D) densities in cardiac ventricles from rats and normal hamsters (NH) as well as in A10 cells. In panel B, the autoradiograph shows the total NOX2 density at the cardiac ventricle level in NHs as well as in A10 cells. In panel D, HK cell extracts were used as positive controls for the NOX4 antibody. Negative controls were performed by incubating the membranes in the presence of the respective antibody blocked with the corresponding NOX peptide from the supplier. The band corresponding to the reference protein GAPDH shows the presence of proteins in the negative control wells.

Article Snippet: In order to confirm equal loading of proteins, the blots were also probed with a mouse monoclonal antibody against GAPDH (6C5) (Abcam, Ontario, Canada).

Techniques: Western Blot, Autoradiography, Negative Control

Figure 2. Decrease in NOX1 density during the development of heart failure in the hereditary cardiomyopathic hamster. (A) Autoradiographs showing NOX1 density in NH, HCMH and rat hearts. (B) Bar graph showing the decrease in NOX1/GAPDH ratio density in HCMH hearts compared to age-matched NH hearts as well as a lower NOX1/GAPDH ratio in rat hearts compared to NH hearts. The values are expressed as mean ± SEM where n is the number of Western blot wells of at least three different animals. *** P <0.001.

Journal: Canadian journal of physiology and pharmacology

Article Title: Increase of NADPH oxidase 3 in heart failure of hereditary cardiomyopathy 1 .

doi: 10.1139/cjpp-2019-0055

Figure Lengend Snippet: Figure 2. Decrease in NOX1 density during the development of heart failure in the hereditary cardiomyopathic hamster. (A) Autoradiographs showing NOX1 density in NH, HCMH and rat hearts. (B) Bar graph showing the decrease in NOX1/GAPDH ratio density in HCMH hearts compared to age-matched NH hearts as well as a lower NOX1/GAPDH ratio in rat hearts compared to NH hearts. The values are expressed as mean ± SEM where n is the number of Western blot wells of at least three different animals. *** P <0.001.

Article Snippet: In order to confirm equal loading of proteins, the blots were also probed with a mouse monoclonal antibody against GAPDH (6C5) (Abcam, Ontario, Canada).

Techniques: Western Blot

Figure 3. Absence of change in NOX2 density during the development of heart failure in the hereditary cardiomyopathic hamster. (A) Autoradiographs showing NOX2 density in NH and HCMH hearts. (B) Bar graph showing that the NOX2/GAPDH ratio does not change in HCMH hearts compared to those from age- matched NHs. The values are expressed as mean ± SEM where n is the number of Western blot wells of at least three different animals.

Journal: Canadian journal of physiology and pharmacology

Article Title: Increase of NADPH oxidase 3 in heart failure of hereditary cardiomyopathy 1 .

doi: 10.1139/cjpp-2019-0055

Figure Lengend Snippet: Figure 3. Absence of change in NOX2 density during the development of heart failure in the hereditary cardiomyopathic hamster. (A) Autoradiographs showing NOX2 density in NH and HCMH hearts. (B) Bar graph showing that the NOX2/GAPDH ratio does not change in HCMH hearts compared to those from age- matched NHs. The values are expressed as mean ± SEM where n is the number of Western blot wells of at least three different animals.

Article Snippet: In order to confirm equal loading of proteins, the blots were also probed with a mouse monoclonal antibody against GAPDH (6C5) (Abcam, Ontario, Canada).

Techniques: Western Blot

Figure 4. Increase in NOX3 density during the development of heart failure in the hereditary cardiomyopathic hamster. (A) Autoradiographs showing NOX3 density in NH, HCMH and rat hearts. (B) Bar graph showing the increase in NOX3/GAPDH ratio in HCMH hearts compared to those from age-matched NHs as well as a higher NOX3/GAPDH ratio in rat hearts compared to NH hearts. The values are expressed as mean ± SEM where n is the number of Western blot wells of at least three different animals.

Journal: Canadian journal of physiology and pharmacology

Article Title: Increase of NADPH oxidase 3 in heart failure of hereditary cardiomyopathy 1 .

doi: 10.1139/cjpp-2019-0055

Figure Lengend Snippet: Figure 4. Increase in NOX3 density during the development of heart failure in the hereditary cardiomyopathic hamster. (A) Autoradiographs showing NOX3 density in NH, HCMH and rat hearts. (B) Bar graph showing the increase in NOX3/GAPDH ratio in HCMH hearts compared to those from age-matched NHs as well as a higher NOX3/GAPDH ratio in rat hearts compared to NH hearts. The values are expressed as mean ± SEM where n is the number of Western blot wells of at least three different animals.

Article Snippet: In order to confirm equal loading of proteins, the blots were also probed with a mouse monoclonal antibody against GAPDH (6C5) (Abcam, Ontario, Canada).

Techniques: Western Blot

Figure 5. Decrease in NOX4 density during the development of heart failure in the hereditary cardiomyopathic hamster. (A) Autoradiographs showing NOX4 density in NH, HCMH and rat hearts. (B) Bar graph showing the decrease in NOX4/GAPDH ratio in HCMH hearts compared to age-matched NH hearts. However, there is no difference in NOX4/GAPDH ratio between rat and NH hearts. The values are expressed as mean ± SEM where n is the number of Western blot wells of at least three different animals. * P <0.05; ** P <0.01; *** P <0.001.

Journal: Canadian journal of physiology and pharmacology

Article Title: Increase of NADPH oxidase 3 in heart failure of hereditary cardiomyopathy 1 .

doi: 10.1139/cjpp-2019-0055

Figure Lengend Snippet: Figure 5. Decrease in NOX4 density during the development of heart failure in the hereditary cardiomyopathic hamster. (A) Autoradiographs showing NOX4 density in NH, HCMH and rat hearts. (B) Bar graph showing the decrease in NOX4/GAPDH ratio in HCMH hearts compared to age-matched NH hearts. However, there is no difference in NOX4/GAPDH ratio between rat and NH hearts. The values are expressed as mean ± SEM where n is the number of Western blot wells of at least three different animals. * P <0.05; ** P <0.01; *** P <0.001.

Article Snippet: In order to confirm equal loading of proteins, the blots were also probed with a mouse monoclonal antibody against GAPDH (6C5) (Abcam, Ontario, Canada).

Techniques: Western Blot

Figure 6. Absence of change in NOX5 density during the development of heart failure in the hereditary cardiomyopathic hamster. (A) Autoradiographs showing the density of NOX5 in NH, HCMH and rat hearts. (B) Bar graph showing that the NOX5/GAPDH ratio does not change in HCMH hearts compared to those from age-matched NHs. This ratio in rat hearts is not different from that in NH hearts. The values are expressed as mean ± SEM where n is the number of Western blot wells of at least three different animals.

Journal: Canadian journal of physiology and pharmacology

Article Title: Increase of NADPH oxidase 3 in heart failure of hereditary cardiomyopathy 1 .

doi: 10.1139/cjpp-2019-0055

Figure Lengend Snippet: Figure 6. Absence of change in NOX5 density during the development of heart failure in the hereditary cardiomyopathic hamster. (A) Autoradiographs showing the density of NOX5 in NH, HCMH and rat hearts. (B) Bar graph showing that the NOX5/GAPDH ratio does not change in HCMH hearts compared to those from age-matched NHs. This ratio in rat hearts is not different from that in NH hearts. The values are expressed as mean ± SEM where n is the number of Western blot wells of at least three different animals.

Article Snippet: In order to confirm equal loading of proteins, the blots were also probed with a mouse monoclonal antibody against GAPDH (6C5) (Abcam, Ontario, Canada).

Techniques: Western Blot